Production of Monoclonal Antibody Against Salmonella H: g,m Flagellar Antigen and Potential Diagnostic Application
Articles > Article · 2010
Authors: Ayşe NALBANTSOY, İSMAİL KARABOZ, Ismet Deliloglu GURHAN
HYBRIDOMA, Journal Indexed in SCI Expanded, International, 2010, vol 29, pp 419-423
Summary
In this study, the flagella antigen was detached at high speed by shaking vigorously with glass pearl beads, from Salmonella enteritidis in a yield of 3.9 mg/mL(-1) after being concentrated with polyethylene glycol (PEG). A monoclonal antibody (MAb), designated D7 clone, was generated from Balb/c mice immunized with Salmonella enteritidis flagella using the conventional hybridoma method. The D7 clone secreting MAb was classified as IgG2a isotype. ELISA analyses of D7 MAb to Salmonella-specific flagella demonstrated that the antibody reacted with H: m. However, Western immunoblot analyses of D7 clone appear to be secreting heavy chain of IgG2a antibody, which was eligible for the diagnosis of Salmonella enteritidis.