Phylogenetic Diversity Analysis and Molecular Identification of Endophytic Fusarium spp. Isolated From Salep (Orchid)
Articles > Article · 2021
Authors: Yüksel GEZGİN
The Journal of Fungus /Mantar Dergisi, Refereed Journals of Other Institutions, International, TR DİZİN (ULAKBİM), 2021, vol 12, pp 163-171
Summary
<p>The endophytic fungi species isolated from Salep (Orchid) roots and tubers, endemic plants of Turkey, their identification using the molecular techniques is utmost important. The aim of the present study was to identify the species of endophytic Fusarium Link isolated from Salep (Orchid). For this purpose, beta-tubulin (ß-tubulin: benA), Internal Transcribed Spacer (ITS) and Translation Elongation Factor (EF) gene regions were amplified by Polymerase Chain Reaction using specific primers. Then, all PCR products were sequenced and compared with the other related sequences in GenBank. The Fusarium isolates were identified to species level as Fusarium redolens Wollenw. (n=7) and Fusarium oxysporum Schlecht. (n=3). The phylogenetic analysis was carried out based on the ß-tubulin, ITS and EF gene sequences. In the present study, Fusarium spp. isolated from Saleps (Orchids) in Turkey were firstly identified by molecular identification methods including amplification of the three different gene region.<br></p>