Purification and Characterization of a Lectin From Bulbs of Cyclamen Mirabile
Makaleler > Tam Makale · 2021
Yazarlar: Ebru KOCADAĞ KOCAZORBAZ, Ayşe Yağmur Azbazdar, Merve Aliakar Öztürk, Esra Menfaatli
Bilecik Şeyh Edebali Üniversitesi Fen Bilimleri Dergisi, Diğer Kurumların Hakemli Dergileri, Uluslararası, TR Dizin (Ulakbim), 2021, cilt 8, ss 1-7
Özet
Lectin from the bulbs of Cyclamen mirabile which is an endemic species of Turkey was successfully isolated by affinity precipitation with alginate in one step. The purified protein produced two bands showing a dimeric structure in SDS-PAGE (13.5 and 14.8 kDa). C. mirabile lectin showed activity and stability in a broad pH scale and kept its haemagglutination activity in the temperature range of 4-40°C. MgCl2 and HgCl2 inhibited the haemagglutination activity of the lectin. In this study, a practical and efficient purification procedure was carried out for C. mirabile lectin by using affinity precipitation with alginate.