In Vitro Biocompatibility Assessment of Lanolin Loaded Cellulose Acetate and Thermoplastic Polyurethane Nanofibers Using L929 Fibroblasts and HS2 Human Keratinocytes
Articles > Article · 2025
Authors: Çiğdem Akduman, Emriye Perrin AKÇAKOCA KUMBASAR, Işık ÖZGÜNEY
Polymer Engineering & Science, Journal Indexed in SCI Expanded, International, WoS, 2025, vol 66, pp 410-424
DOI: 10.1002/pen.70222
Summary
ABSTRACT This study explores the potential of lanolin‐loaded nanofiber mats as an innovative material for skin‐contact biomedical applications, summarizing their development and biocompatibility assessment. Lanolin‐loaded cellulose acetate (CA) and thermoplastic polyurethane (TPU) nanofibers, produced via electrospinning, exhibited promising morphological properties and were compared. Following sterilization, extracts of these nanofibers were prepared at various concentrations (100%, 50%, 25%, 12.5%, and 6.25% v/v) and evaluated for cytotoxicity using the MTT assay. Two cell lines were utilized for in vitro testing: the L929 mouse fibroblast cell line, as recommended by ISO 10993‐5 to represent mammalian systems, and the HS2 human keratinocyte cell line. Results indicated that lanolin positively influenced cell viability and proliferation, with TPU/Lanolin nanofibers achieving the best outcomes. Based on these results, analyses were continued with the TPU/lanolin nanofibers, which showed better performance. Optimization studies revealed that a 3–5 h collection period and a lanolin concentration of 30% (w/w) yielded optimal results. The addition of lanolin minimally impacted air permeability, which remained low across all samples, without compromising water permeability. The most promising configuration was found to be TPU/30Lanolin‐5h. These findings suggest that, upon further validation, such lanolin‐loaded nanofiber mats hold promise for future use in healthcare products designed to promote skin comfort and healing.