The Importance of PFGE and Rapd Methods in Determination of Philogenetic Relation among E. Coli Sustains Isolated from Extraintestinal Infection and whose Virulance Factors are Defined
Makaleler > Tam Makale · 2020
Yazarlar: Başak BEDİR, AymenAziz Khalid, Hassan T.S. SHATUB, Shadman Sadiq, Fatih KOKSAL, Ousman Bajinka
Annals of Microbiology and Infectious Diseases, Uluslararası, 2020, cilt 3, ss 1-3
DOI: 10.22259/2637-5346.0304001
Özet
In this study, 155 Escherichia coli strains were isolated to investigatethe association between PFGE and RAPD.The freeze-thaw method was applied for RAPD.The results showed PGFE had a higher discrimination power than RAPD-PCR.With the both methodsjust13(30.9%)samples showed clonal relation, the relation might be a random mutation rather than epidemic since the strains were isolated from the in-patient ofdifferent clinics.The study concluded that RAPD method is more advantageous than PEGE method in terms of the phylotyping and interpretation of obtained results.