Carbon nanotube composite as novel platform for microbial biosensor
Makaleler > Tam Makale · 2007
Yazarlar: Ulku ANIK, Suna TİMUR, Dilek ODACI, Briza PEREZ, Salvador ALEGRET, Arben MERKOCI
ELECTROANALYSIS, SCI Expanded İndekslerine Giren Dergi, Uluslararası, 2007, cilt 19, ss 893-898
Özet
The presented work includes the development of a microbial biosensor based on a carbon-nanotube epoxy composite (CNTEC) platform used as supporting electrode for cell immobilization. For this purpose, cells of Pseudomonas fluorescens were immobilized on the surface of the CNTEC electrode by means of gelatin which was then cross linked with glutaraldehyde. After optimization of experimental parameters like cell amount, pH and temperature, the system was calibrated for glucose. From the calibration graph the linear range was estimated as 0.5 - 4.0 mM with a response time of 100 s. Furthermore, substrate specificity and operational stability were investigated. Finally, the results that were obtained with CNTEC electrodes were compared with conventional graphite epoxy composite electrode (GECE) and as a result, higher current values (2 to 3 folds) were observed with CNTEC microbial biosensor.