Experimental profiling and in silico validation of autophagy and apoptosis-related genes in murine lung cancer and fibroblast cells
Makaleler > Tam Makale · 2026
Yazarlar: Berrin ÖZDİL BAY, Yasemin Adalı, Yasemin Berberoğlu, Irmak Tutak, Aylin Koseler, Hüseyin AKTUĞ
Ege Tıp Dergisi, Uluslararası, 2026, cilt 65, ss 55-67
DOI: 10.19161/etd.1763314
Özet
Aim: Autophagy and apoptosis are key programmed cell death pathways that play a critical role in cellular homeostasis. Dysregulation of these pathways is frequently associated with cancer development and progression. Comparing the molecular signatures of tumor and normal cells may provide novel insights into the survival mechanisms adopted by cancer cells.Objective: The objective of this study was to compare the expression profiles of key autophagy- and apoptosis-related genes between murine lung carcinoma cells and non-malignant fibroblasts and to assess their regulatory interactions using in silico analyses.Materials and Methods: KLN-205 murine lung carcinoma cells and mouse skin fibroblasts (MSF) were cultured under standard conditions. The mRNA expression levels of Atg7, SQSTM1, Beclin-1, Caspase-3, Caspase-8, Caspase-9, Bax, and Bcl-2 were quantified by RT-qPCR. Normalized data were analyzed using 2-ΔΔCT method, and statistical differences between groups were evaluated using unpaired t-tests. In silico analyses were performed to explore broader expression patterns and molecular interactions, including principal component analysis (PCA) and the construction of protein–protein interaction (PPI) networks using the STRING database.Results: A significant downregulation of Atg7 was observed in KLN-205 cells compared to MSF (p