Lens Lipid Peroxides and Glutathione Concentrations in Diabetic Cataract
Articles > Article · 1997
Authors: D Özmen, I Mutaf, B Özmen, J Mentes, O Bayindir
Annals of Clinical Biochemistry: International Journal of Laboratory Medicine, International, WoS, 1997, vol 34, pp 190-192
DOI: 10.1177/000456329703400211
Summary
This study aims to explore the role of reactive oxygen radicals in the genesis of diabetic cataract. Lipid peroxide (LPO) concentrations in senile (n = 30) and diabetic (n = 14) cataractous lenses, were determined as thiobarbituric acid-reactive substances (TBARS) by a method modified from Satoh and Yagi, and reduced glutathione (GSH) concentrations were measured according to Beutler. Lens LPO levels (mean, SD; nmol TBARS/g protein) were significantly higher in diabetics (107.54, 18.12) than senile cataractous subjects (53.54, 15.48) (P < 0.0001). Lens GSH levels (mean, SD; nmol/g protein) showed no significant difference between diabetics (4.29, 2.05) and senile cataractous subjects (4.68, 3.12). These results suggest that free radical damage is more effective in the genesis of diabetic cataract than in senile cataract.