Sol-gel encapsulated glucose oxidase arrays based on a pH sensitive fluorescent dye
Makaleler > Tam Makale · 2011
Yazarlar: Nimet YILDIRIM, Dilek ODACI, Gulsiye Ozturk, Serap Alp, Yavuz Ergun, Kay DORNBUSCH, Karl-Heinz FELLER, Suna TİMUR
DYES AND PIGMENTS, SCI Expanded İndekslerine Giren Dergi, Uluslararası, 2011, cilt 89, ss 144-148
DOI: 10.1016/j.dyepig.2010.10.003
Özet
Optical glucose oxidase (GOx) arrays based on pH sensitive fluorescent dye (2-(4-tolyl)-4-[4-(1,4,7,10-tetraoxa-13-azacyclopentadecyl)benzylidene]-5-oxazolone) (CPO) has been constructed. The arrays were prepared by spotting of CPO and GOx together with tetraethoxysilane (TEOS)/Chitosan (CHIT) mixture via a microarrayer. After optimization studies, analytical characterization of enzyme arrays were carried out. The fluorescence intensity of the system was linearly correlated to glucose concentration in the range of 1.0-30.0 mM (in potassium phosphate buffer; 2.5 mM at pH 7.0). Furthermore, the developed arrays were used to analyze glucose in some beverages and HPLC was used as a reference method for independent glucose analysis. (C) 2010 Elsevier Ltd. All rights reserved.