α-Amylase Monitoring by a Novel Amperometric Biosensor Based on Au Electrode: Its Optimization, Characterization, and Application
Articles > Article · 2011
Authors: Umut MENGÜLLÜOĞLU, Çağrı ALTUĞ, H. Deniz ERTUGRUL, Abdulkerim YILDIZ, E. Melis EKICI, Erhan DİNÇKAYA
Artificial Cells, Blood Substitutes, and Biotechnology, Journal Indexed in SCI Expanded, International, WoS, 2011, vol 40, pp 97-102
DOI: 10.3109/10731199.2011.597757
Summary
A low-cost and sensitive amperometric biosensor was developed for the determination of α-amylase activity. The biosensor was constructed by immobilizing glucose oxidase-gelatin via glutaraldehyde on the Au electrode surface. Measurements were carried out chronoamperometrically at -0.7 V. Several parameters such as glucose oxidase activity, gelatin amount, and glutaraldehyde percentage for cross-linking were optimized. Optimum pH, optimum temperature, repeatability, and storage stabilities of the biosensor were identified. Under the optimum experimental conditions, a linear calibration curve was obtained for α-amylase between 0.819 and 13.110 U/ml. Sample analyses were carried out by detecting α-amylase activities in baker's yeast samples.