Adsorption of lysozyme from aqueous solutions by a novel bentonite-tyrptophane (Bent-Trp) microcomposite affinity sorbent
Makaleler > Tam Makale · 2015
Yazarlar: Tulden Kalburcu, Ahmet TABAK, NEVRA Ozturk, Nalan Tuzmen, Sinan AKGÖL, BÜLENT ÇAĞLAR, ADİL DENİZLİ
JOURNAL OF MOLECULAR STRUCTURE, SCI Expanded İndekslerine Giren Dergi, Uluslararası, 2015, cilt 1083, ss 156-162
DOI: 10.1016/j.molstruc.2014.11.055
Özet
This study deals with developing bentonite-tryptophan (Bent-Trp) microcomposite affinity sorbent (38-105 gm) for lysozyme adsorption from aqueous solutions. The structural analysis results of Bent-Trp microcomposites obtained from X-ray diffraction (XRD), Fourier Transform Infrared (FTIR), thermal (TG-DTG/DTA) and elemental analysis techniques clearly pointed out that the pseudo-biospecific affinity ligand L-Tryptophan was penetrated into interlayer space of the bentonite by diplacing water molecules and an elemental analysis of immobilized L-Tryptophan for nitrogen was 541.3 mu mol g(-1) bentonite. The effects of initial concentration, pH, and temperature on the adsorption efficiency of this sorbent were also studied in a batch system. The maximum amount of lysozyme adsorption from aqueous solution was determined as 365.16 mg/g at pH 10.0 and 1.25 mg/mL initial concentration of lysozyme, while the non-specific adsorption of lysozyme was very low (4.21 mg g(-1) sorbent). We concluded that Bent-Trp microcomposite affinity sorbents could be repeatedly applied for lysozyme adsorption without significant losses in the adsorption capacity. (C) 2014 The Authors. Published by Elsevier B.V.