A Biosensor for the Determination of β-galactosidase Activity: A Different Viewpoint on Biosensors
Makaleler > Tam Makale · 2011
Yazarlar: Mustafa Kemal Sezginturk, Erhan DİNÇKAYA
Artificial Cells, Blood Substitutes, and Biotechnology, SCI Expanded İndekslerine Giren Dergi, Uluslararası, Wos, 2011, cilt 39, ss 281-288
DOI: 10.3109/10731199.2011.560119
Özet
β-galactosidase splits lactose into glucose and galactose. Because of its biotechnological interest, we presented a biosensor system in order to monitor β-galactosidase activity. Immobilization steps of the biosensor were identified by cyclic voltammograms and electrochemical impedance spectroscopy. β-galactosidase was voltammetrically detected at about +150 mV (vs. Ag/AgCl) in citrate buffer solution (0.05 M, pH 4.8). The linear response for β-galactosidase detection was in the range of 0.0118 U mL(-1)to 0.47 U mL(-1)and a shorter response time of ∼50 s. Our results demonstrated the biosensor's electrochemical properties and analytical characteristics were very useful and effective for monitoring of β-galactosidase activity.