Optical Mapping of the Fusarium oxysporum f. sp. melongenae Genome
Diğer yayınlar > Diğer · 2021
Yazarlar: Duygu ATEŞ, Hacer Handan Altinok, Muhammed Bahattin TANYOLAÇ
Diğer, Uluslararası, 2021,
Özet
Abstract Optical mapping approaches are widely preferred and applied in different branches of genomic studies because of their accuracy, low cost, and high efficiency. In the current study, a sequence orientation of the Fusarium oxysporum f. sp. melongenae (FOMG) genome that is deposited in GenBank NCBI under accession number MPIL00000000 was used as the reference genome, which we checked with Bionano Genomics (BNG) optical mapping approaches. BNG optical mapping produced 103 contigs, the longest of which was 3.05 Mb. The N50 value of BNG contigs is 0.85 Mb. The sequences of the FOMG reference genome and BNG optical map mainly match each other. Results obtained in the current study indicate that BNG optical mapping can be used to construct complete and gapless assemblies of the FOMG genome. It also can be applied to validate a previous genome assembly.