Double fluorescence assay via a beta-cyclodextrin containing conjugated polymer as a biomimetic material for cocaine sensing
Articles > Article · 2017
Authors: Mustafa Arslan, Tulay Yilmaz SENGEL, Emine GÜLER ÇELİK, Zinar Pınar GÜMÜŞ, EBRU ALDEMİR, Huseyin Akbulut, Hakan COŞKUNOL, Suna TİMUR, Yusuf Yağcı
POLYMER CHEMISTRY, Journal Indexed in SCI Expanded, International, 2017, vol 8, pp 3333-3340
DOI: 10.1039/c7py00420f
Summary
A double fluorescence mu-well assay that exploits a novel conjugated polymer containing cyclodextrin (CD) as the key component is reported. For the construction of the cocaine bioassay, poly(p-phenylene) with CD units in the main-chain and poly(ethylene glycol) side chains (PPP-CD-g-PEG) was first prepared by Suzuki coupling polymerization and coated on each well as a biomimetic material. Although the poly-phenylene backbone is responsible for the fluorescence properties without an additional fluorophore, PEG and CD provide water solubility and selective complexation with cocaine, respectively. A cocaine antibody was used as a secondary recognition compound after labelling with quantum dots (QDs). Most notably, we show that the two-color fluorescence nature of the assay facilitates double measurement from the same sample and the described strategy can be adapted to various sensing systems.