Estradiol protects adipose tissue–derived stem cells against H2O2‐induced toxicity
Makaleler > Tam Makale · 2012
Yazarlar: Dilek TAŞKIRAN, Vedat EVREN
Journal of Biochemical and Molecular Toxicology, SCI Expanded İndekslerine Giren Dergi, Uluslararası, Wos, 2012, cilt 26, ss 301-307
DOI: 10.1002/jbt.21421
Özet
Oxidative stress is associated with various pathophysiological processes, including cell survival, adhesion, apoptosis, and cancer. In the present study, we aimed to evaluate the effects of H(2)O(2)-induced toxicity on adipose tissue-derived stem cells (ADSCs) and whether 17β-estradiol (E(2)) has protective effects on these cells. ADSCs derived from adult Sprague-Dawley rats were pretreated with different doses of E(2) for 24 h and then exposed to 200 µM H(2)O(2) for 4 h. Incubation of ADSCs with H(2)O(2)-decreased cell viability in a concentration-dependent fashion (p < 0.0001), whereas pretreatment of these cells with E(2) significantly reversed toxicity (p < 0.05), inhibited apoptotic changes, and decreased lipid peroxidation (p < 0.0005). Our findings suggest that E(2) protects ADSCs from oxidative-induced cell death, and therefore, it may be used to improve the survival rate and regenerative capacity of stem cells.