Boronate affinity nanoparticles for RNA isolation
Makaleler > Tam Makale · 2015
Yazarlar: Aykut TOPRAK, Cansu GORGUN, Cansu Ilke KURU, Ceren TURKCAN, Murat Uygun, Sinan AKGÖL
MATERIALS SCIENCE & ENGINEERING C-BIOMIMETIC AND SUPRAMOLECULAR SYSTEMS, SCI Expanded İndekslerine Giren Dergi, Uluslararası, 2015, cilt 50, ss 251-256
DOI: 10.1016/j.msec.2014.11.033
Özet
In this presented paper, boronic acid incorporated poly(HEMA) based nanoparticles were synthesized for RNA adsorption. For this purpose, poly(HEMA) nanopartides were synthesized by using the surfactant free emulsion polymerization technique. Then, nanoparticles were modified with 3-(2-imidazoline-1-yl)propyl(triethoxysilane) (IMEO) and functionalized with phenylboronic acid (PBA). Prepared nanoparticles were characterized with SEM, FUR and zeta-size. Optimum RNA adsorption conditions were investigated with different pHs, temperatures and initial RNA concentrations in order to determine the maximum RNA adsorption onto poly(HEMA)-IMEO-PBA nanoparticles. It was also studied that, synthesized nanoparticles could be used for 5 successive reuses and adsorption capacity of the nanoparticles decreased only about 5% at the end of the 5 cycles. (C) 2014 Elsevier B.V. All rights reserved.