Evaluation of a multi-parameter biomarker set for oxidative damage in man: Increased urinary excretion of lipid, protein and DNA oxidation products after one hour of exercise
Makaleler > Tam Makale · 2004
Yazarlar: Hilmi ORHAN, B Van Holland, BETTY KRAB, JANINE MOEKEN, NPE Vermeulen, PETER HOLLANDER, JHN Meerman
FREE RADICAL RESEARCH, SCI Expanded İndekslerine Giren Dergi, Uluslararası, 2004, cilt 38, ss 1269-1279
DOI: 10.1080/10715760400013763
Özet
The objective of the present. study was to evaluate a comprehensive set of urinary biomarkers for oxidative damage to lipids, proteins and DNA, in man. Eighteen moderately trained males (mean age 24.6 +/- 0.7) exercised 60 min at 70% of maximal O-2 uptake on a cycle ergometer. Urine fractions for 12 h were collected 1 day before, and for 3 consecutive days after exercise. As biomarkers of lipid peroxidation, 8 aldehydes (i.e. propanal, butanal, pentanal, hexanal, heptanal, octanal, nonanal and malondialdehyde-MDA)and acetone were analyzed in urines by gas chromatography with electron capture detection (GC-ECD).