Rapid Fibroblast Cell Culture Characterization with Impedimetric Label‐free Heat Shock Protein – 47 Biosensor
Articles > Article · 2020
Authors: Duygu Harmanci, ZİHNİ ONUR UYGUN, Ayse Kocak Sezgin, Cenk Demirdover, Ferhan GİRGİN SAĞIN, Gul Akdogan
Electroanalysis, Journal Indexed in SCI Expanded, International, WoS, 2020, vol 32, pp 2310-2315
Summary
Abstract In this study, for the first time in literature, an impedimetric biosensor technology was developed to determine Heat Shock Protein‐47 (HSP‐47) for the characterization of primary skin fibroblast cells. Gold nanoparticle electrodes (GNPE) were modified with amino‐functionalized graphene oxide layers and anti‐HSP‐47 were immobilized on this layer. The performance of the biosensor was tested with real samples as cell lysates. The low detection limit (LOD) and the linear detection limit (LOQ) of the biosensor were calculated as 9.47 pg/mL and 3.23 pg/mL, respectively. Linear measurement range was obtained between 10–160 pg/mL HSP‐47. The reproducibility of the biosensor was good.