Magnetic Nanoparticle-Based Electrochemical Sensing Platform Using Ferrocene-Labelled Peptide Nucleic Acid for the Early Diagnosis of Colorectal Cancer
Makaleler > Tam Makale · 2022
Yazarlar: Simge Balaban Hanoglu, Ezgi Man, Duygu Harmanci, Serife Tozan Ruzgar, Serdar Sanli, Nazim Arda Keles, Atakan Ayden, Bilge Guvenc Tuna, Ozgul Duzgun, Omer Faruk Ozkan, Soner Dogan, Faezeh GHORBANIZAMANI, Hıchem MOULAHOUM, Emine GÜLER ÇELİK, Serap EVRAN, Suna TİMUR
BIOSENSORS-BASEL, SCI Expanded İndekslerine Giren Dergi, Uluslararası, Scopus, 2022, cilt 12, ss 736-736
DOI: 10.3390/bios12090736
Özet
Diagnostic biomarkers based on epigenetic changes such as DNA methylation are promising tools for early cancer diagnosis. However, there are significant difficulties in directly and specifically detecting methylated DNA regions. Here, we report an electrochemical sensing system based on magnetic nanoparticles that enable a quantitative and selective analysis of the methylated septin9 (mSEPT9) gene, which is considered a diagnostic marker in early stage colorectal cancer (CRC). Methylation levels of SEPT9 in CRC samples were successfully followed by the selective recognition ability of a related peptide nucleic acid (PNA) after hybridization with DNA fragments in human patients’ serums and plasma (n = 10). Moreover, this system was also adapted into a point-of-care (POC) device for a one-step detection platform. The detection of mSEPT9 demonstrated a limit of detection (LOD) value of 0.37% and interference-free measurement in the presence of branched-chain amino acid transaminase 1 (BCAT1) and SRY box transcription factor 21 antisense divergent transcript 1 (SOX21-AS1). The currently proposed functional platform has substantial prospects in translational applications of early CRC detection.