In Vitro Drug Release Kinetics and Antioxidant Activity of Metformin-Loaded Niosomes
Makaleler > Tam Makale · 2025
Yazarlar: Emine Esin Çalışkan, Yalçın Çelik Aydın, Emine Nur ÖZBEK ATALAY, Ilayda Alcın, Mustafa Emrah KILINÇ, Günay YETİK ANACAK, Emel Öykü ÇETİN UYANIKGİL
ACS Omega, Uluslararası, Wos, 2025, cilt 10, ss 58225-58237
Özet
Metformin is an antidiabetic drug that is widely used in the treatment of type 2 diabetes mellitus (T2DM) and is known to reduce oxidative stress. Drug-loaded niosomes enhance the cellular uptake of drugs, resulting in improved antioxidant effects. In this study, we formulated metformin-loaded niosomes, aiming to enhance cellular drug uptake and augment antioxidant effects. The particle size, polydispersity index, and zeta potential values were found to be 153.8 nm, 0.449, and -9.32 mV respectively. Morphological observations conducted through scanning electron microscopy (SEM) provided insights into the distinctive structure of the niosomes. Entrapment efficiency of drug-loaded niosomes was determined to be 68%. In vitro drug release studies, performed by using the dialysis bag method, exhibited a release profile consistent with Hixson-Crowell kinetics. After characterization of the formulations, the antioxidant activity of metformin-loaded niosomes on Pyrogallol-induced reactive oxygen species (ROS) formation in the mouse brain was compared with metformin treatment alone. ROS formation was measured by a luminol-lucigenin chemiluminescence assay. Metformin-loaded niosomes significantly reduced ROS formation compared to metformin treatment alone. Metformin-loaded niosomes offer potential for enhanced antioxidant effects and bioavailability by increasing cellular uptake of metformin.