Enzymatic synthesis of I-125/131 labeled 8-hydroxyquinoline glucuronide and in vitro/in vivo evaluation of biological influence
Articles > Article · 2011
Authors: Reyhan YESILAGAC, Perihan ÜNAK, Emin İlker MEDİNE, Çiğdem İÇHEDEF, Turkan Ertay, Fazilet Zümrüt BİBER MÜFTÜLER
APPLIED RADIATION AND ISOTOPES, Journal Indexed in SCI Expanded, International, 2011, vol 69, pp 299-307
DOI: 10.1016/j.apradiso.2010.10.027
Summary
8-Hydroxyquinoline (8-OHQ) is a long-known molecule which due to its metal-complexation ability is frequently used for analysis. It is also called oxine. Oxine and derivatives have been investigated to process antitumor and antimicrobial activities. 8-Hydroxyquinolyl-glucuronide (8-OHQ-Glu) was enzymatically synthesized using microsome preparates separated from Hutu-80 cells, labeled with I-125 to perform a radionuclide labeled prodrug and investigated of its biological affinities on Hutu-80 (human duodenum intestinal adenocarcinoma), Caco-2 (human colorectal adenocarcinoma), Detroit 562 (human pharynx adenocarcinoma) cells and ACBRI 519 (primary human small intestine epithelial cells) in this work. UDP-glucuronyl transferase (UDPGT) rich microsome preparates, which are used for glucuronidation in enzymatic synthesis, were extracted from Hutu-80 cells.