A Ready‐to‐Use Electrochemical Kit Design for the Diagnosis of Single Nucleotide Polymorphisms
Makaleler > Tam Makale · 2013
Yazarlar: Yasin Ugur KAYRAN, Dilşat ARIKSOYSAL, Burçin KAYMAZ, Buket KOSOVA, Mehmet OZSOZ
Electroanalysis, SCI Expanded İndekslerine Giren Dergi, Uluslararası, Wos, 2013, cilt 25, ss 2668-2676
Özet
Abstract In this study, for the first time a model electrochemical kit was constructed for the detection of a functional polymorphism in catechol‐O‐methyl transferase (COMT) gene which is important for diagnosis of neuropsychiatric disorders as Alzheimer disease. The disposable pencil graphite electrode (PGE) is designed as a “kit” and the probe DNA covered PGE can detect single nucleotide polymorphisms (SNPs) from real samples based on the guanine oxidation signal even after 5 months of kit preparation (150 days durability).The detection limit (S/N=3) of the biosensor was calculated as 1.18 pmol of synthetic target sequence and 6.09×105 molecules of real samples in 30 min detection time.