Development of a Label Free IGE Sensitive Aptasensor Based on Electrochemical Impedance Spectrometry
Articles > Article · 2010
Authors: Pınar KARA, Buket Meric, Mehmet Ozsoz
Combinatorial Chemistry & High Throughput Screening, Journal Indexed in SCI Expanded, International, WoS, 2010, vol 13, pp 578-581
DOI: 10.2174/1386207311004070578
Summary
A sensitive aptamer based electrochemical biosensor to detect human immunoglobulin E (IgE) is presented in this study. 5' Biotin labeled 45 mer DNA aptamer sequence was immobilized onto streptavidin coated graphite surfaces. Interaction between human IgE and DNA aptamer was monitored by Electrochemical Impedance Spectrometry (EIS) in a 0.48nM detection limit of IgE. EIS analysis are based on electron transfer resistance (Rct) in the presence of 5mM [Fe(CN)6]3-/4-.