Suppression of STAT5A increases chemotherapeutic sensitivity in imatinib-resistant and imatinib-sensitive K562 cells
Makaleler > Tam Makale · 2010
Yazarlar: Buket KOSOVA, Burçin KAYMAZ, Huseyin Atakan Ekiz, Zeynep Cakir, Nur SELVİ GÜNEL, Aysegul DALMIZRAK, Melis Kartal, UFUK GÜNDÜZ, Yusuf Baran
Leukemia & Lymphoma, SCI Expanded İndekslerine Giren Dergi, Uluslararası, Wos, 2010, cilt 51, ss 1895-1901
DOI: 10.3109/10428194.2010.507830
Özet
STAT proteins are cytoplasmic transcription factors that are involved in the regulation of numerous cellular activities such as cell growth, differentiation, and survival. In this study, we aimed to identify the expression pattern of STAT genes in imatinib-sensitive and -resistant K562 cells, and further, to reveal the effects of STAT5A siRNA knockdown on cell growth and apoptosis induction. The XTT cell proliferation assay showed that both sensitive and resistant K562 cells were sensitized to imatinib upon transfection with STAT5A siRNA. Caspase-3 enzyme activity was increased significantly in both cells. These results may open up new opportunities to overcome chemotherapeutic resistance in leukemia.