Electrochemical sensing of label free DNA hybridization related to breast cancer 1 gene at disposable sensor platforms modified with single walled carbon nanotubes
Makaleler > Tam Makale · 2012
Yazarlar: Chen-zhong Lİ, Hakan KARADENİZ, Ece CANAVAR, Kadriye Arzum ERDEM GÜRSAN
ELECTROCHIMICA ACTA, SCI Expanded İndekslerine Giren Dergi, Uluslararası, 2012, cilt 82, ss 137-142
DOI: 10.1016/j.electacta.2012.05.057
Özet
The electrochemical monitoring of DNA hybridization related to specific sequences on breast cancer 1 (BRCA1) DNA by using singlewalled carbon nanotube (SWCNT) based screen printed graphite electrodes (SPEs) was performed in this study. After the microscopic characterization of SWCNT-SPE, the optimization of assay was studied. The development of screen printing process combined with nanomaterial based disposable sensor technology with sensitivity, selectivity and reproducibility, has a great opportunity for DNA detection using differential pulse voltammetry (DPV) by measuring the guanine oxidation signal observed at +1.00 V in the presence of DNA hybridization between BRCA1 probe and its complementary target. The detection limit estimated for signal to noise ratio = 3 corresponds to 378.52 nM target concentration in the 401,1 samples. The voltammetric results on BRCA1 DNA hybridization were also complemented with electrochemical impedance spectroscopy (EIS). (C) 2012 Elsevier Ltd. All rights reserved.