Aptasensor for Impedimetric Detection of Lysozyme
Book chapter > Chapter Other Book · 2022
Authors: Ece Eksin, Muhammed Hüseyin ŞENTÜRK, Kadriye Arzum ERDEM GÜRSAN
Methods in Molecular Biology, Chapter Other Book, Springer Science+Business Media, B, International, 2022, pp 197-204, United States of America
DOI: 10.1007/978-1-0716-2695-5_15
Summary
The impedimetric detection of a protein, lysozyme (LYS), was carried out herein by aptamer-immobilized single-use pencil graphite electrodes (PGEs). The aptamer was immobilized onto electrochemically activated surface of electrode without using any chemical agents, or any types of nanomaterials. Cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) techniques were applied to analyze the electrochemical behavior of unmodified PGE and aptamer immobilized PGE. The interaction of aptamer with its target protein, LYS, was then investigated by EIS. The limit of detection for LYS was found to be 1.44 μg/mL (equals to 100.65 nM). The developed aptasensor specific to LYS presented high selectivity against to bovine serum albumin and thrombin.