Production ofTrichodermamicropropagules as a biocontrol agent in static liquid culture conditions by using an integrated bioreactor system
Articles > Article · 2019
Authors: Isik Coban, Sait SARGIN
Biocontrol Science and Technology, Journal Indexed in SCI Expanded, International, WoS, 2019, vol 29, pp 1197-1214
DOI: 10.1080/09583157.2019.1672621
Summary
In this study, we optimised the conditions for the production of micropropagules of Trichoderma harzianum EGE-K38 in static liquid culture in Modified Czapec Medium (MCM) containing 8 g/L glucose in an integrated tray bioreactor system designed by our research group. Incubation temperature, air flow rate, inoculum spore concentration, inoculation size, medium volume and the use of spores or agar plugs containing mycelia as inoculum were individually studied as one factor at a time. The maximum micropropagule count was 5.2 ± 0.2 × 109 cfu/mL and dry cell weight was 17 ± 2 g/L. For the subsequent drying processes, the maximum drying yield percentage ((viable micropropagule counts after drying/viable cells before drying)*100) after drying of micropropagules was 23.30% (cfu/cfu). Results obtained from our integrated tray bioreactor system showed that static liquid culture fermentation offers potential for industrial scale fungal BCAs production.