Evaluating the Sensitivity of Conventional PCR for Detecting Pathogenic Prototheca Species
Articles > Article · 2025
Authors: Enes Göksal, İnci TÜNEY KIZILKAYA
Süleyman Demirel Üniversitesi Fen Bilimleri Enstitüsü Dergisi, Refereed Journals of Other Institutions, International, TR DİZİN (ULAKBİM), 2025, vol 29, pp 569-575
DOI: 10.19113/sdufenbed.1694623
Summary
This study aimed to evaluate the molecular detection limit (Limit of Detection, LOD) of two pathogenic Prototheca strains using conventional PCR and re-amplification PCR (re-PCR) methods. Genomic DNA samples from Prototheca ciferrii (SAG 2063) and Prototheca bovis (LZ-5) were subjected to 1:2 serial dilutions and evaluated by PCR. In conventional PCR, amplification was observed up to approximately 9.1𝚡10-4 ng/µL for P. ciferrii and 5.34𝚡10-6 ng/µL for P. bovis. Re-PCR allowed the detection of amplification products at even lower DNA concentrations in both strains. These findings demonstrate that Prototheca species can be detected at very low DNA concentrations using molecular techniques and that re-PCR may enhance diagnostic sensitivity.