Multiparametric approach to assess the disease severity and progression of cutaneous leishmaniasis infection
Makaleler > Tam Makale · 2022
Yazarlar: Shumaila Naz, Aiman Aroosh, Naeem Raza, Arshad Islam, Yusuf ÖZBEL, Seray TÖZ, Haroon Ahmed, Shahid Waseem
Acta Tropica, SCI Expanded İndekslerine Giren Dergi, Uluslararası, Scopus, 2022, cilt 235, ss 106659-106659
DOI: 10.1016/j.actatropica.2022.106659
Özet
The pathophysiology of Cutaneous Leishmaniasis (CL), an infection caused by Leishmania tropica (L. tropica) and Leishmania major (L. major) is primarily determined by inflammation-mediated immune cells. The immune response mainly depends on cells and molecules related to T-cells that influence susceptibility and disease development. Understanding the immunological mechanisms that cause tissue injury or lesion healing is critical for developing appropriate treatment strategies. In the present study, T-cells profile and cell-free mitochondrial DNA (CF mt-DNA) were investigated in CL patients infected with L. tropica (n = 34) and L. major (n = 2) and compared with non-infected healthy controls (n = 20). There was a significant (p<0.0001) difference between CD4+ T-cells among L. tropica and L. major CL-infected groups as compared to control while no significant difference (p = 0.8597) was found in the percentages of CD8+ T-cells. When L. tropica and L. major CL-infected individuals were compared to controls, the levels of IL-4 and expression of CF mt-DNA were significantly higher (p<0.0001). Higher levels of CF mt-DNA were detected in CL patients, irrespective of the infective Leishmania species. We proposed that the levels of CF mt-DNA and IL-4 in CL-infected individuals can be used to determine the disease progression. A better understanding of these biomarkers and evaluation of the immune responses in CL patients might benefit the development of vaccines and immunotherapies.