Association of the Toll-like receptor 4 and NOX4 gene and protein levels in asthmatic patients with metabolic syndrome: A case–control study
Makaleler > Tam Makale · 2023
Yazarlar: Sevda Ghoushouni, Akbar Sharifi, Venus Zafari, Habib Zarredar, Ensiyeh Seyedrezazadeh
Journal of Research in Medical Sciences, Uluslararası, Wos, 2023, cilt 28, ss 11-11
Özet
Background: Understanding the contributing of influence inflammatory biomarkers in asthmatic patients with metabolic syndrome is more important. Whereby, the present study considering the important association of NADPH oxidase4 (NOX4) and Toll- like receptor4 (TLR4) in the respiratory inflammatory responses in asthmatic patients with metabolic syndrome (AS-MetS) and asthmatic (AS) patients. Materials and Methods: In this case-control study, 30 AS and 34 AS-MetS patients were enrolled. The Peripheral blood mononuclear cells (PBMCs) mRNA and protein levels of TLR4 and NOX4 were measured by qRT-PCR and western blot, respectively. Then their correlation was evaluated. Results: The significant down-regulation of mRNA and protein PBMCs expression levels of TLR4 were observed in the AS-MetS group in comparison to AS one ( P =0.03), but the NOX4 expression was non-significant. Additionally, the significant correlation was exhibited between mRNA expression levels of NOX4 and TLR4 in both AS-MetS ( r = 0.440, P =0.009) and AS groups ( r =0.909, P =0.0001). The association between TLR4 mRNA level and triglyceride in AS-MetS group ( r =0.454, P =0.008,) and also white blood cells (WBC) in AS group ( r = -0.507, P =0.006,) were significant. Conclusion: The metabolic syndrome can significantly influence the expressions of TLR4 in AS-MetS. This study indicated that TLR4 and NOX4 altogether may provide valuable molecular knowledge of their relation with metabolic syndrome criteria for finding major pathways in different phenotype of asthma.