Differences and Similarities between Colorectal Cancer Cells and Colorectal Cancer Stem Cells: Molecular Insights and Implications
Makaleler > Tam Makale · 2023
Yazarlar: Derya ERIŞIK, Berrin ÖZDİL BAY, EDA AÇIKGÖZ, CEMİLE SİNEM ASKER ABDİKAN, TAHA KADİR YESİN, HÜSEYİN AKTUĞ
ACS Omega, SCI Expanded İndekslerine Giren Dergi, Uluslararası, Wos, 2023, cilt 8, ss 30145-30157
Özet
cells) were cultured for 72 h. The levels of E-cadherin, KLF4, p53, p21, p16, cyclin D2, HDAC9, and P300 protein expression were determined using immunohistochemistry staining. The migration of cells was assessed by employing the scratch assay technique. Additionally, the scanning electron microscopy method was used to examine the morphological features of the cells, and their peripheral/central elemental ratios were compared with the help of EDS. Furthermore, a Muse cell cycle kit was utilized to determine the cell cycle analysis. The HT-29 CSC group exhibited high levels of expression for E-cadherin, p53, p21, p16, cyclin D2, HDAC9, and P300, whereas KLF4 was found to be high in the HT-29. The two groups did not exhibit any statistically significant differences in the percentages of cell cycle phases. The identification of specific CSC characteristics will allow for earlier cancer detection and the development of more effective precision oncology options.