Dipeptidyl peptidase IV production by solid state fermentation using alternative fungal sources
Makaleler > Tam Makale · 2012
Yazarlar: Gaye ÖNGEN ÖZGEN, Sait SARGIN, Ozlem ÜSTÜN, Ceren Kutlu, Mesut Yucel
TURKISH JOURNAL OF BIOLOGY, SCI Expanded İndekslerine Giren Dergi, Uluslararası, 2012, cilt 36, ss 665-671
DOI: 10.3906/biy-1201-26
Özet
The present work was carried out for the production of dipeptidyl peptidase IV (DPP IV; EC 3.4.14.5) using Aspergillus, Penicillium, and Rhizopus strains under solid state fermentation conditions. Response surface methodology was applied for the optimization of the selected operational variables (corn flour, initial moisture content, and cultivation time) for DPP IV activity as the response. The optimal parameters of DPP IV activity for the independent variables, namely the amount of corn flour (% w/w), initial moisture content (% w/w), and cultivation time (days), were evaluated to be 2.44%, 60.85%, and 4.69 days, respectively, using Aspergillus awamori T116. The response for these results was also shown to be in very close agreement with the experimental data.