The protective effect of resveratrol against dentin bonding agents-induced cytotoxicity
Makaleler > Tam Makale · 2015
Yazarlar: Çiğdem ATALAYIN ÖZKAYA, Güliz ARMAGAN, Sibel KONYALIOĞLU, Hande KEMALOĞLU, Huseyin Tezel, Zeynep ERGÜCÜ, Ayşegül KESER, Taner DAĞCI, Banu Onal
Dental Materials Journal, SCI Expanded İndekslerine Giren Dergi, Uluslararası, Wos, 2015, cilt 34, ss 766-773
DOI: 10.4012/dmj.2015-079
Özet
This study was designed to evaluate the cytotoxicity of four dentin bonding agents and the effects of an antioxidant addition. Group A: G-aenial Bond, Group B: Optibond All in One, Group C: Gluma Self Etch and Group D: Clearfil S(3) Bond were added to the medium using extract method. The cells were cultured with or without resveratrol (RES) addition. MTT, reactive oxygen species (ROS), DCF, Comet and 8-OHdG measurements were performed. The agents had a dose-dependent (1:1>1:10>1:20) cytotoxic effect. Considering 1:10 concentration; Group D at 1 h (p<0.01) and Group B and D at 24 h had the weakest cytotoxic effect (p<0.05). After RES addition, the highest cell viability was determined in Groups B+RES and D+RES at 1 h and in Groups A+RES and B+RES at 24 h (p<0.01). The dentin bonding agents induced ROS production and DNA damage regarding to their composition. However, RES addition decreased the indicated parameters.