A Novel Alternative for the Treatment of Diabetic Foot Wounds: A Three Dimensional Porous Dermal Matrix
Bildiriler > Tam Metin Bildiri
Yazarlar: Evren Homan GÖKÇE, Sakine TUNCAY TANRIVERDİ, Ipek Eroglu, Nicolas Tsapis, Göksel GÖKÇE, Elias Fattal, Kevser Özgen ÖZER
World Congress on Recent Advances in Nanotechnology, , , Uluslararası, Tam Metin Bildiri, Scopus
DOI: 10.11159/nddte17.121
Özet
Diabetic wounds tend to heal slowly and the healing process can be very complicated due to polymicrobial infection and heavy exudate formation which place patients at a higher risk for limb amputation [1].The investigations in wound care field revealed bioactive dermal matrices produced from a variety of proteins of extracellular matrix (ECM) have the ability to promote the healing process [2].In addition reduction/termination of the persistent inflammation and elimination of free radicals by the introduction of an antioxidant could be an important strategy to improve healing [3].Therefore a novel alternative for the treatment of diabetic foot wounds consisting of a three dimensional collagen-laminin porous dermal matrix impregnated with resveratrol (RSV)-loaded hyaluronic acid (HA) and dipalmitoylphosphatidylcholine (DPPC) microparticles was evaluated.Characterization, in vitro release, microbiological, ex vivo and in vivo studies were performed.Spherical microparticles of 30.2±0.3 μm were obtained with a RSV encapsulation efficacy of 98.7%.Scanning electron microscopy (SEM) and confocal laser scanning microscopy showed that particles were well dispersed in the dermal matrix from the surface to deeper layers.Collagenase degraded dermal matrix, however the addition of RSV loaded microparticles delayed the degradation time.The release of RSV was sustained and reached 70% after 6 h.Histological changes and antioxidant parameters in different treatment groups were investigated in full-thickness excision diabetic rat model.The highest healing score was obtained with the dermal matrix impregnated with RSV-microparticles with an increased antioxidant activity.Collagen-laminin dermal matrix with RSV microparticles can be an effective and safe option for the treatment of diabetic wounds requiring long recovery.