GC/MS Analysis of Alkaloids in Galanthus fosteri Baker and Determination of Its Anticholinesterase Activity
Makaleler > Tam Makale · 2018
Yazarlar: Ahmet EMİR, Ceren EMİR, Buket BOZKURT, Nehir SOMER
Turkish Journal of Pharmaceutical Sciences, Uluslararası, Scopus, 2018
DOI: 10.4274/tjps.26056
Özet
Objectives: Amaryllidaceae alkaloids are well known for their wide range of pharmacological activities.Galanthamine, an Amaryllidaceae alkaloid, is effective, selective, reversible, and competitive cholinesterase inhibitor marketed under the different commercial name in several countries for the treatment of Alzheimer's disease.This work was aimed at studying the alkaloid profiles of the aerial parts and bulbs of both flowering and fruiting periods of Galanthus fosteri Baker (Amaryllidaceae), as well as analyzing their inhibitory activities on both acetylcholinesterase (AChE) and butyrylcholinesterase (BuChE), for the first time. Materials and Methods:The alkaloid profiles of the four samples were determined by means of Gas Chromatography-Mass Spectrometry (GC-MS), and acetylcholinesterase and butyrylcholinesterase inhibition assays were performed by the modified Ellman method.Results: Totally, twenty-two compounds with mass spectral characteristics of Amaryllidaceae alkaloids were detected in the extracts.Significant acetylcholinesterase and butyrylcholinesterase inhibitory activities were observed in the tested samples (IC50 between 0.189 and 91.23 μg/mL). Conclusion:This study shows that G. fosteri, collected from Akdağ, Amasya (Turkey), has a potential of being the source of diverse chemical structures of Amaryllidaceae alkaloids which have cholinesterase inhibitory property.