A hederagenin-type triterpene saponin, sumbulianoside a from Cephalaria sumbuliana and its potent immunomodulatory activity against seasonal flu virus H3N2
Makaleler > Tam Makale · 2021
Yazarlar: Huseyin Abaci, Gulsah Akagac, Ayşe NALBANTSOY, Nazlı SARIKAHYA
NATURAL PRODUCT RESEARCH, SCI Expanded İndekslerine Giren Dergi, Uluslararası, 2021, cilt 36, ss 2495-2503
DOI: 10.1080/14786419.2021.1910691
Özet
A new hederagenin-type triterpene saponin; hederagenin 28-O-beta-D-galactopyranosyl-(1 -> 6)-beta-D-glucopyranosyl ester named sumbulianoside A (1), together with twelve known saponins were isolated from the n-butanol extract of Cephalaria sumbuliana (Caprifoliaceae) from which, one known saponin, dipsacus saponin A (2) was isolated, for the first time from Cephalaria species. The structures of the isolated compounds were elucidated by 1 D and 2 D NMR and HRESIMS analyses. Cytotoxic activities were investigated on A549, Hela, PANC1, SHSY5Y cells and non-cancerous cell HEK293 by MTT method and immunomodulatory activities were evaluated against activated H3N2 seasonal virus in whole blood by measuring IL-4, IFN-gamma, IL-1 beta cytokine level with ELISA. According to the cytotoxicity results, compounds 1 and 2 did not possess significant cytotoxicity, while only compound 2 induced significant IL-4 production (** p<.001) against H3N2 showing a possible Th2 response and antibody production. All compounds had no effect on cytokine release (p > 0.5).