Epigenetic Regulation of Prostate Normal and Cancer Cell Lines
Articles > Article · 2017
Authors: Ceren Gonen Korkmaz, Buket REEL, Mehmet Zuhuri ARUN, Gokce Yildirim‐Buharalioglu
The FASEB Journal, International, WoS, 2017, vol 31
DOI: 10.1096/fasebj.31.1_supplement.996.7
Summary
Epigenetic investigates gene expression modifications not due to DNA sequence alterations. Gene expression modifications appear with packing of DNA with variety of chromatine structures. The most studied forms of epigenetic phenomenon are DNA methylation and histone modifications. These pathways are connected with each other and reversible. DNA methylation reported genes: 36B4 (as control), AR, HN1, PTEN, KLLN, STAMP1, STAMP2 primers were used and methylation status had been confirmed at normal WPMY‐1 prostate and lymhp node metastasis LNCaP and brain metastasis DU145 cancer cell lines, respectively. Histone modification which close the gene expression is histone deacetylation had been reversed by Trichostatin A(TSA). It is reported that inhibition of methylation and deacetylation in a serial way result with succesful gene activation, performed with 5‐Azacitidine and TSA incubation at the project. In the recent years, epigenetic base had been shown for many diseases that research and development for epigenetic disorders won successful treatment cases. Preclinic and clinic levels of candidate drugs are on the way. Support or Funding Information EGE UNIV. SCIENTIFIC RESEARCH PROJECT