Parametric continuous feed stream design to fine-tune fed-batch bioreactor performance: recombinant human growth hormone production in Bacillus subtilis
Makaleler > Tam Makale · 2016
Yazarlar: Guzide Calik, Pınar KOCABAŞ, Hande Afsar, PINAR ÇALIK, Tuncer H. Ozdamar
JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY, SCI Expanded İndekslerine Giren Dergi, Uluslararası, 2016, cilt 91, ss 2740-2750
DOI: 10.1002/jctb.4864
Özet
BACKGROUND: Design principles of a fed-batch bioreactor system to fine-tune bioreactor performance by increasing production and productivity are investigated. To enhance the production and productivity in fed-batch fermentation of recombinant human growth hormone (rhGH) by Bacillus subtilis, this work develops a continuous feed stream (CFS) design method with the parameters, cultivation time to switch batch-to fed-batch operation, so called transition time, t(T), and specific growth rate, mu.