Impedimetric detection of in situ interaction between anti-cancer drug bleomycin and DNA
Makaleler > Tam Makale · 2013
Yazarlar: Kadriye Arzum ERDEM GÜRSAN, Gulsah Congur
International Journal of Biological Macromolecules, SCI Expanded İndekslerine Giren Dergi, Uluslararası, Wos, 2013, cilt 61, ss 295-301
DOI: 10.1016/j.ijbiomac.2013.07.012
Özet
Surface confined interaction of anti-cancer drug bleomycin (BLM) with nucleic acids: single stranded and double stranded DNA was investigated herein by using electrochemical impedance spectroscopy (EIS) technique in combination with a graphite sensor technology. The experimental conditions were optimized: such as, dsDNA concentration, BLM concentration and interaction time. The main features of impedimetric DNA biosensor, such as its detection limit and the repeatability, were also discussed. The in situ interaction of BLM with dsDNA was also tested impedimetrically in the absence or presence of other chemotherapeutic agents, such as mitomycin C (MC) and cis-platin (cis-DDP) for testing the selectivity. (C) 2013 Elsevier B.V. All rights reserved.