The effects of Hericium erinaceus extracts on cell viability and telomerase activity in MCF-7 cells
Makaleler > Tam Makale · 2023
Yazarlar: Duygu Gencalp Rustem, Hikmet Hakan AYDIN, Erbil Kalmis, Hüsniye KAYALAR, Handan Ak
TURKISH JOURNAL OF BIOCHEMISTRY-TURK BIYOKIMYA DERGISI, SCI Expanded İndekslerine Giren Dergi, Uluslararası, Wos, 2023, cilt 48, ss 298-302
Özet
Objectives: This study aims to determine the effects of Hericium erinaceus extracts on cell viability and the effects of H. erinaceus water extract on the telomerase activity of MCF-7 cells. H. erinaceus is an edible mushroom widely used in traditional Chinese medicine. Although its various therapeutic properties, the literature has not yet submitted evidence about H. erinaceus for its effects on the telomerase activity of MCF-7 cells.Methods: MCF-7 cells were treated with ethanol, ethanol-water, ether, ethyl acetate, methanol-water, and water extracts to determine the effects on cell viability using the WST8 method. The TeloTAGGG Telomerase PCR ELISA kit was used to assess telomerase activity.Results: The water extract was determined to be the most efficient extract to decrease cell viability. The water extract's half-maximal inhibitory concentration was 250 mu g/mL at 72 h. It is found that H. erinaceus has no statistically significant effect compared to positive control on reducing telomerase activity. We found a statistically significant difference in telomerase activity % between H. erinaceus water extracts and negative control (p<0.05).Conclusions: Consequently, these differences in telomerase activity are a significant association rather than inferring action. It is considered that water extract shows its cell viability inhibition effects through different mechanisms.