Bioassay-guided isolation studies on mesophilic Actinomycete M-33–5 possessing antibacterial and cytotoxic activities
Makaleler > Tam Makale · 2009
Yazarlar: M Urgen, F Kocabaş, A Nalbantsoy, O Serçinoğlu, E Hames-Kocabaş, Ataç UZEL, E Bedir
Planta Medica, Uluslararası, Wos, 2009, cilt 75
Özet
One hundred and twenty six mesophilic Actinomycete cultures were isolated from Aegean Region of Turkey. The antimicrobial activities of pure isolates were tested by using agar-plaque method. Based on high antimicrobial activity versus methicillin resistant Staphylococcus aureus (MRSA) and Escherichia coli 232 (E. coli), the isolate M-33–5 was selected for bioactivity-guided isolation. Fermentation followed by solvent partition studies (H 2 O-EtOAc, H 2 O-n-BuOH) showed that the highest activity was present in EtOAc extract. By using chromatographic methods, two bioactive compounds were isolated. Structures of the active metabolites were determined to be griseusin A ( 1 ) and 4′-deacetyl griseusin A ( 2 ) by spectral methods [1]. MIC values of 4′-deacetyl griseusin A were less than 1µg/ml versus MRSA and E. coli. The cytotoxicities of the EtOAc extract and 4′-deacetyl griseusin A were also evaluated by the MTT assay using two human cancer cell lines (L-929, HeLa). Both showed potent cytotoxic activities against aforementioned cell lines. Acknowledgement: This study was supported by Turkish Scientific and Technological Research Council of Turkey (Project No: SBAG-2746). References: [1] Tsuji, N. et al. (1976) Tetrahedron 32:2207–2210.