Quantitative analysis of lycorine in Galanthus alpinus Sosn. var. alpinus by HPLC-DAD
Makaleler > Tam Makale · 2018
Yazarlar: Ceren EMİR, Nehir SOMER
\n Volume: 48, Issue: 2\n 32-37\n \n, Uluslararası, 2018
Özet
DOI:10.5152/IstanbulJPharm.2018.416835In this study, a reversed-phase, highpressure liquid chromatography (RP-HPLC) method was applied in the quantitativeanalysis of lycorine in the aerial parts and bulbs of Galanthus alpinus Sosn.var. alpinus collected from Rize during the flowering and fruiting seasons. Asimple method to extract lycorine from the plant specimens was performed, using pre-packed columns withdiatomaceous earth (Extrelut®). The chromatographic separation was achievedemploying an isocratic system with a mobile phase including trifluoroaceticacid-water-acetonitrile (0.01:95:5) measured at a flow rate of 1 mL/min using adiode array detector (DAD). The lycorine content in the bulbs during theflowering and fruiting seasons was detected as 0.01576 and 0.02351 %,respectively. Also, validation studies showed that the method was specific,accurate and precise.