Paper-based electrode assemble for impedimetric detection of miRNA
Makaleler > Tam Makale · 2020
Yazarlar: Ece Eksin, HİLAL TORUL, Ece Yarali, UĞUR TAMER, Pagona Papakonstantinou, Kadriye Arzum ERDEM GÜRSAN
Talanta, SCI Expanded İndekslerine Giren Dergi, Uluslararası, Wos, 2020, cilt 225, ss 122043-122043
DOI: 10.1016/j.talanta.2020.122043
Özet
In the present work, a paper-based electrode assemble was developed and implemented to detect target microRNA 155 (miRNA 155) via electrochemical impedance spectroscopy (EIS) measurements. In this concept, gold nanoparticles (AuNPs) modified paper based electrode assemble system (AuNP-PE) was designed, and characterized by scanning electron microscopy (SEM), cyclic voltammetry (CV) and EIS measurements. The impedimetric detection of miRNA 155 was performed by measuring the fractional change at the charge transfer resistance (R-ct). The detection limits were found as 33.8 nM in PBS and 93.4 nM in fetal bovine serum (FBS) medium, respectively. The selectivity of the proposed assay was tested against to non-complementary (NC) and mismatch (MM) miRNA sequences in the presence of mixture sample containing miRNA:NC (1:1) and miRNA: MM (1:1) in PBS (pH 7.40) or FBS. The analytical performance and the selectivity of impedimetric biosensor were also tested in FBS.