Histidine modified montmorillonite: Laccase immobilization and application to flow injection analysis of phenols
Articles > Article · 2013
Authors: Didem SONGURTEKIN, Esra Evrim YALÇINKAYA, Didem AG, Muharrem SELECI, Dilek ODACI, Suna TİMUR
Applied Clay Science, Journal Indexed in SCI Expanded, International, WoS, 2013, vol 86, pp 64-69
DOI: 10.1016/j.clay.2013.10.014
Summary
The preparation, characterization and application of histidine (His) modified montmorillonite (His-Mt) in electrochemical biosensor fabrication were reported. His was used as a modifier to enhance immobilization capability of Mt for the biomolecules. Mt modifications were followed by X-ray diffraction, Fourier transform infrared spectroscopy, zeta potential and thermal gravimetry analysis. After a successful immobilization of Laccase enzyme on the His-Mt matrix, covered on a glassy carbon electrode, application in catechol biosensing was investigated in detail. Optimization, analytical characteristics, repeatability, and operational stability studies were evaluated using both batch and flow injections analysis configurations. (C) 2013 Elsevier B.V. All rights reserved.