Genetic diversity and major spoligotype families of drug-resistant Mycobacterium tuberculosis clinical isolates from different regions of Turkey
Articles > Article · 2007
Authors: Riza DURMAZ, Thierry ZOZIO, Selami GUNAL, Akgun YAMAN, Cengiz ÇAVUŞOĞLU, Cengiz GUNEY, Christophe SOLA, Nalin RASTOGI
Infection, Genetics and Evolution, Journal Indexed in SCI Expanded, International, WoS, 2007, vol 7, pp 513-519
DOI: 10.1016/j.meegid.2007.03.003
Summary
To highlight the transmission rate and major phyloPnetic clades of drug-resistant Mycobacterium tuberculosis isolates, a total of 200 drug-resistant strains isolated in four different regions of Turkey (Marmara n = 8 1; Mediterranean n = 39; Aegean n = 42; East Anatolia n = 38), were typed by spoligotyping and IS6110-restriction fragment length polymorphism (RFLP). The major spoligotyping-defined shared-types (STs) and corresponding lineages were, ST 41 (22.5%, LAM7-TUR), ST53 (19.5%, ill-defined T super-family), ST 50 (6.5%, Haarlem 3), ST 1261 (4.5%, LAM7-TUR), ST 47 (3.5%, Haarlem 1), as well as two STs that belonged to undefined clades (ST 284, 3%, and ST 2067, 2.5%). The global distribution of major M. tuberculosis lineages among drug-resistant strains was as follows: T super-family (29%), Latin-American & Mediterranean (33.5%), Haarlem (14%), and the S lineage (3%). A high number of strains (n = 29, 14.5%) showed patterns that did not fall within major clades described so far. A combination of spoligotyping and IS61 10-RFLP fingerprinting methods resulted in a final clustering rate of 38.5% and a recent transmission rate of 25.5%. Our results underline the highly diverse nature of drug-resistant tuberculosis in our study population. as well as its ongoing transmission with lineages that are specific to these regions, the most predominant being the LAM7-TUR lineage which shows an enhanced phylogeographical specificity for Turkey. (c) 2007 Elsevier B.V. All rights reserved.