Chitosan modified graphite electrodes developed for electrochemical monitoring of interaction between daunorubicin and DNA
Makaleler > Tam Makale · 2018
Yazarlar: Gulsah Congur, Ece Eksin, Kadriye Arzum ERDEM GÜRSAN
Sensing and Bio-Sensing Research, SCI Expanded İndekslerine Giren Dergi, Uluslararası, Scopus, 2018, cilt 22, ss 100255-100255
DOI: 10.1016/j.sbsr.2018.100255
Özet
Chitosan (CHIT) modified single-use electrodes were developed for the first time in the present study and applied for electrochemical monitoring of anticancer drug-DNA interaction. Under this aim, pencil graphite electrode (PGE) was used as the biosensor platform and the modification of PGEs using biopolymer, CHIT was performed by passive adsorption process. Microscopic and electrochemical characterizations of CHIT modified PGEs were performed. An anticancer drug, Daunorubicin (DNR) was analyzed by using both PGEs and CHIT-PGEs. Moreover, the effect of CHIT modification on biosensor development upon to the sensitivity of voltammetric detection of DNR and DNA was also investigated. The interaction between DNA and DNR was then performed and accordingly, the voltammetric measurements were performed before/after interaction process by using differential pulse voltammetry (DPV) technique. • Chitosan (CHIT) modified disposable pencil grahite electrodes (PGEs) were developed. • An application about detection of drug-DNA interaction by CHIT-PGEs was shown. • Daunorubicin and DNA interaction was monitored by CHIT-PGEs as the first study. • Voltammetric analysis which was fast, reliable and sensitive were performed. • CHIT-PGEs and PGEs were also compared in a manner of detection performance.