IN VITRO INHIBITORY ACTIVITY OF BERBERIS VULGARIS L. AGAINST LEISHMANIA TROPICA PROMASTIGOTES
Makaleler > Tam Makale · 2019
Yazarlar: AHMET ÖZBİLGİN, Hüsniye KAYALAR, ibrahim cavus, cenk durmuşkaya, ÜMİT TOKTAŞ, Cumhur GÜNDÜZ
Universal Journal of Pharmaceutical Research, Diğer Kurumların Hakemli Dergileri, Uluslararası, Scopus, 2019, cilt 4, ss 22-24
Özet
In the present study it was aimed to determine the in vitro antileishmanial activity of Berberis vulgaris L. against Leishmania tropica promastigotes. The aerial parts of Berberis vulgaris were collected from Spil Mountain, Manisa. The ethanolic extract of the plant material was prepared. The consecutive concentrations of the plant extract (25-100µg/ml) were set for in vitro antileishmanial assays. In addition to in vitro inhibitory activities against Leishmania tropica promastigotes, the cytotoxicity of the plant extract was also measured by WST-1 Cell proliferation assay. The percentages of parasite inhibition in the presence of B. vulgaris ethanol extract in comparison with glucantime reference group at time interval of 12-72 hours were observed between 88.0 and 100.0 %. The plant extract was found to have cytotoxic activity with 444, 81±2, 12 µg/ml IC50 value.This is the first study that involves the in vitro antileishmanial activity of B. vulgaris which is wildly growing in Manisa, Turkey. Initial results demonstrated that the ethanolic extract of B. vulgaris gave promising results and it could be used as an antileishmanial agent in future.