Cutaneous leishmaniasis caused by Leishmanıa infantum in Turkey: reports of two cases diagnosed with genotyping and protein fingerprinting
Makaleler > Tam Makale · 2012
Yazarlar: G. Culha, I. Akyar, F. Yildiz Zeyrek, Cumhur GÜNDÜZ, O. KURT, I. OSTAN, Seray TÖZ, T. Kocagoz, Y. Ozbel, A. Ozbilgin
International Journal of Infectious Diseases, SCI Expanded İndekslerine Giren Dergi, Uluslararası, Wos, 2012, cilt 16, ss e397-e397
DOI: 10.1016/j.ijid.2012.05.531
Özet
Background: Cutaneous leishmaniasis (CL) is endemic in mainly the southeastern regions of Turkey. The causative agent in most CL cases is predominantly Leishmania tropica, whereas L. infantum is mainly identified in patients with visceral leishmaniasis. However, there are few reports from Turkey that indicates L. infantum as the causative agent of CL. Here, we report two CL cases from Osmaniye and Hatay provinces located in eastern Mediterranean region in Turkey. Methods: Normal 0 21 false false false TR X-NONE X-NONE MicrosoftInternetExplorer4 Samples obtained from the skin lesions of the patients were initially stained with Giemsa and cultivated in NNN medium. Examination of the smears and culture materials revealed Leishmania amastigotes and promastigotes, respectively. The promastigotes (MHOM/TR/2012/CBU15 and MHOM/TR/2012/MK05) obtained from the culture of both patients were used for further molecular analyses. Real-Time PCR analysis targeting the ITS-1 region in the SSU of rRNA were conducted to determine the Leishmania species of the cases. Results: The results demonstrated that both samples were L. infantum, which was confirmed by protein fingerprinting analyses conducted by MALDI-TOF. Conclusion: It is concluded that application of molecular methods determine the causative agents of infectious diseases more precisely than conventional methods, which is essential for correct diagnosis, effective treatment and prevention of severe epidemics (The present study is funded by TUBITAK-Project No: 111S179).