The effect of cyclosporin a via P53, MDM2 and PIK3CA gene expressions in glioblastoma multiforme cells
Makaleler > Tam Makale · 2010
Yazarlar: Çığır BİRAY AVCI, Zeynep Özlem Doǧan, Nezih Oktar, Tayfun Dalbasti, Sunde Yilmaz, Sinem Numanoǧlu, Taner AKALIN, Cumhur GÜNDÜZ
Journal of Neurological Sciences, SCI Expanded İndekslerine Giren Dergi, Uluslararası, Scopus, 2010, cilt 27, ss 170-177
Özet
Objective: It is aimed to evaluate the effect of cyclosporine A in glioma cells via p53, MDM2 and PIK3CA gene expressions. Material Methods: U87MG cells are treated with 10, 30 and 60 μM CsA during 72 hour in time and dose dependent manner. The cell group that was not treated by any agent was approved of the control group. During 72 hour, total RNA was isolated from the cells which were collected every 24 hour and complementary DNA was synthesized from these cells. Dose and time dependent manner variation analysis of p53, MDM2 and PIK3CA gene expressions were performed by Real-Time Online RT-PCR. Relative analyses of these genes were achieved by rating the genes' expressions to GAPDH. Results: p53 expression showed an increase in 10[xM dose of CsA in time and dose dependent manner and the rise of expression was four times at the 48th hour. MDM2 expression was increased approximately 1,5 times. When PIK3CA expression was evaluated, it was seen that, in the 24th hour, 30 [xM dose of CsA showed 67% suppressed expression according to control group. Conclusions: Due to the results of the performed study by the well-known drug CsA, it is found that up-regulation of functional p53 tumor suppressor and induction of apoptosis triggered by p53 can be effective strategy in the induction of glioma cell death. These data carry a critical weight in the pharmacological explorations related with gliomas.