The effects of cell recycling on the production of 1,3-propanediol by Klebsiella pneumoniae
Makaleler > Tam Makale · 2013
Yazarlar: Fatma Gizem AVCI, Damla HÜCCETOĞULLARI, Nuri AZBAR
Bioprocess and Biosystems Engineering, SCI Expanded İndekslerine Giren Dergi, Uluslararası, Wos, 2013, cilt 37, ss 513-519
DOI: 10.1007/s00449-013-1018-z
Özet
The effects of both biomass age and cell recycling on the 1,3-propanediol (1,3-PDO) production by Klebsiella pneumoniae were investigated in a membrane-supported bioreactor using hollow-fiber ultrafiltration membrane module in two separate experiments. It was determined that older cells have a negative effect on 1,3-PDO production. The concentrations of by-products, such as acetic acid and ethanol, increased in cultures with older cells, whereas the concentrations of succinic acid, lactic acid and 2,3-butanediol decreased. The effect of cell recycling was comparatively studied at a cell recycling ratio of 100 %. The results showed that cell recycling had also negative effects on 1,3-PDO fermentation. It was hypothesized that both cell recycling and biomass age caused metabolic shifts to undesired by-products which then inhibited the 1,3-PDO production. On the other hand, the use of hollow-fiber ultrafiltration membrane module was found to be very effective in terms of removal of cells from the fermentation broth.